◆ BrdU

◆ Mouse BrdU Monoclonal Antibody

◆ Anti-mouse Secondary Antibody

PART 1：5-溴脫氧尿嘧啶核苷（BrdU）

產品信息：

化學特性：

產品描述：

5-溴脫氧尿嘧啶核苷（BrdU）是胸腺嘧啶的衍生物，常用于標記活細胞中新合成的DNA，可代替胸腺嘧啶選擇性整合到復制細胞中新合成的DNA中（細胞周期S期）。這種摻入可以穩定存在，隨著DNA復制進入子細胞中。BrdU 特異性抗體可以用于檢測BrdU的摻入，從而判斷細胞的增殖能力。Brdu與抗體的結合需要DNA變性，通常用酸或加熱的方法進行處理。Brdu在標記濃度范圍內既不具有放射活性也沒有骨髓毒性，因此也常用于研究腫瘤細胞的體內增殖狀況。

產品應用：

v         體外實驗：

1）   為檢測EBs增殖，在培養基中加入30 μM BrdU^[1]。

2）   為檢測Shh對EGL細胞增殖的影響，用重組的Shh-N培養細胞，其中含有10 μM BrdU^[2]。

3）   為檢測小鼠胚胎成纖維細胞（MEFs）的增殖，細胞在含有10 μM BrdU的培養基中孵育4h，anti-BrdU 單克隆抗體（1:10）孵育1h, 用wesern blottin檢測^[3]。

4）   為檢測大鼠SMCs細胞增殖，用含有0.5% FCS的DMEM培養基培養細胞，加入10 μM BrdU ^[4]。

v         體內實驗：

1）   在小鼠全組織包埋實驗中，給小鼠腹腔注射100 μg/gm體重的BrdU，用1:200 的anti-BrdU抗體孵育48-72h^[5]。

2）   為標記有絲分裂活躍的細胞，給大鼠腹腔注射50 mg/kg BrdU（PBS溶解）。用抗BrdU小鼠單克隆抗體（1:100）孵育組織切片^[6]。

3）   為檢測大鼠腦組織中BrdU標記的核酸，給hx 大鼠每天腹腔注射50 mg/kg 體重的BrdU，用小鼠anti-BrdU抗體（1:400）孵育樣品^[7]。

參考文獻：

[1] Carmeliet, P., et al. Role of HIF-1α in hypoxiamediated apoptosis, cell proliferation and tumour angiogenesis. Nature, 394: 485-490(1998).

[2] Wallace, V.A., et al. Purkinje-cell-derived Sonic hedgehog regulates granule neuron precursor cell proliferation in the developing mouse cerebellum. Current Biology, 9:445–448(1999).

[3] Jacobs, J.L., et al. The oncogene and Polycombgroup gene bmi-1regulates cell proliferation and senescence through the ink4a locus. Nature, 397(14): 164-168(1999).

[4] Janssens, S., et al. Human Endothelial Nitric Oxide Synthase Gene Transfer Inhibits Vascular Smooth Muscle Cell Proliferation and Neointima Formation After Balloon Injury in Rats. Circulation. 97:1274-1281(1998).

[5] Doetsch, F., et al. Lack of the Cell-Cycle Inhibitor p27Kip1 Results in Selective Increase of Transit-

Amplifying Cells for Adult Neurogenesis. The Journal of Neuroscience, 22(6): 2255-2264 (2002).

[6] Kuhn, H.G., et al. Epidermal Growth Factor and Fibroblast Growth Factor-2 Have Different Effects on Neural Progenitors in the Adult Rat Brain. The Journal of Neuroscience, 17(15): 5820- 5829(1997).

[7] Maria, A.I., et al. Peripheral Infusion of IGF-I Selectively Induces Neurogenesis in the Adult Rat Hippocampus. The Journal of Neuroscience, 20(8):2896-2903(2000).

PART 2：Mouse BrdU Monoclonal Antibody

產品信息（現貨供應）：

抗體信息：

引用文獻：

[1] George-Weinstein, M., Foster, R.F., Gerhardt, J.V., and Kaufman, S.J. (1993). In vitro and in vivo expression of 7 integrin and desmin define the primary and secondary myogenic lineages. Dev. Biol. 156, 209-229.

[2] Dominov, J.A., Dunn, J.J., and Miller, J.B. (1998). Bcl-2 expression identifies an early stage of myogenesis and promotesclonal expansion of muscle cells. J. Cell Biol. 142, 537-544.

[3] Orris, A.S., Banicky, L.C., and Wiens, D.J. (1999). Isotretinoin alters morphology, polarity, and motility of neural crestcells in culture. Reprod. Tox. 13(1), 45-52.

[4] Vicaro-Abejon, C., Yusta-Boyo, M.J., Fernandez-Moreno, C., and de Pablo, F. (2003). Locally born olfactory bulb stem cells proliferate in response to insulin-related factors and require endogenous insulin-like growth factor-I for differentiation into neurons and glia. J. Neurosci. 23(3), 895-906.

[5]Yusta-Boyo, M.J., Gonzalez, M.A., Pavon, N., Martin, A.B., de la Fuente, R., Garcia-Castro, J., de Pablo, F., Moratalla, R.,

[6]Bernad, A., and Vicario-Abejon, C. (2004). Absence of hematopoiesis from transplanted olfactory bulb neural stem cells.Eur. J. Neurosci. 19, 505-512.

[7]Garelli, A., Rotstein, N.P., and Politi, L.E. (2006). Docosahexaenoic acid promotes photoreceptor differentiation without altering Crx expression. Invest. Ophthalmol. Vis. Sci. 47(7), 3017-3027.

[8]Otaegi, G., Yusta-Boyo, M.J., Vergano-Vera, E., Mendez-Gomez, H.R., Carrera, A.C., Abad, J.L., Gonzalez, M., de la Rosa, E.J.,

[9]Vicaro-Abejon, C., and de Pablo, F. (2006). Modulation of the PI 3-kinase-Akt signalling pathway by IGF-I and PTEN regulates the differentiation of neural stem/precursor cells. J. Cell Sci. 119, 2739-2748.

[10]Vergano-Vera, E., Yusta-Boyo, M.J., de Castro, F., Bernad, A., de Pablo, F., and Vicario-Abejon, C. (2006). Generation of GABAergic and dopaminergic interneurons from endogenous embryonic olfactory bulb precursor cells. Development 133,4367-4379.

[11]Francisco-Morcillo, J., Hidalgo-Sánchez, M., Martín-Partido, G., (2006). Spatial and temporal patterns of proliferation and differentiation in the developing turtle eye. Brain Res. 1103, 32-48.

[12]Shibasaki, K., Takebayashi, H., Ikenaka, K., Feng, L., and Gan, L. (2007). Expression of the basic helix-loop-factor Olig2 in the developing retina: Olig2 as a new marker for retinal progenitors and late-born cells. Gene Expr. Patterns 7, 57-65.

[13]Tamiya, S., Liu, LH, and Kaplan, H.J. (2010). Epithelial-Mesenchymal transtition and proliferation of retinal pigment epithelial cells initiated upon loss of cell-cell contact. Invest. Ophthalmol. Vis. Sci. 51(5), 2755-2763.

[14] Gerbe, F., van Es, J.H., Makrini, L., Brulin, B., Mellitzer, G., Robine, S., Romagnolo, B., Shroyer, N.F., Bourgaux, J.-F., Pignodel, C., Clevers, H., and Jay, P. (2011). Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium. J. Cell Biol. 192(5), 767-780.

[15] Martinez-de Luna, R.I., Kelly, L.E., and El-Hodiri, H.M. (2011). The retinal homeobox (Rx) gene is necessary for retinal regeneration. Dev. Biol. 353, 10-18.

Part 3：Anti-mouse secondary antibody

Jackson ImmunoResearch Inc.是美國著名的二抗生產商。JIR公司的二抗以種類豐富（物種齊全，標記多樣，應用廣泛），性價比高，供貨迅捷而深受國際科研學者和工業客戶的青睞。JIR公司不僅堅持提供傳統標記的二抗（如酶標記，FITC，羅丹明，Cy系列等），而且始終與最前沿的染料生產商合作，以最快的速度供應新型染料標記二抗。目前市場上最受歡迎，口碑最好的莫屬DyLight^TM和Alexa Fluor^?系列熒光染料，從客戶需求和公司發展戰略出發，JIR公司將在繼續提供部分DyLight^TM標記二抗的基礎上，著重推出Alexa Fluor^?熒光標記二抗。作為DyLight熒光染料的完美替代物，Alexa Fluor^?同樣具有以下優點甚至表現更好：熒光強度高，光耐受性（抗淬滅性）強，水溶性好，儲存不會產生沉淀，pH值不敏感，儀器兼容性廣。另外從藍光到遠紅外范圍，能覆蓋多種顏色且分辨率高，非常適合多標實驗。

山羊抗小鼠酶標二抗：

山羊抗小鼠熒光二抗：

遠紅外-紅外山羊抗小鼠熒光二抗（熒光WB，與LiCor Odyssey imaging systems兼容）：